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Leukocyte adhesion assay Human leukocytes were isolated from peripheral blood of volunteers (under written consent) by using Ficoll-Paque Plus (Amersham Pharmacia Biotech, NJ, USA) according to manufactures guidelines and incubated with 2'7'-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (2 M