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Cutis Aplasia Cutis aplasia, or aplasia cutis congenita, is a condition that is present at birth and characterized by an absence of skin

doi: 10.1016/j.ctrv.2014.04.004 166 MinettM

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Supplementation of polysorbate 80 (P-80), span 80 (S-80) or polysorbate 20 (P-20) Pure P-80 (Merck KGaA, catalogue number: 8.22187.0500, lot number: S736868771), S-80 (Croda) or P-20 (Merck KGaA, Catalogue number: 8.22184.0500, Lot number: S7514484809) was added into the liquid NGM agar medium before autoclaving to obtain a final concentration of 100 M, 200 M, or 400 M

and Barros et al., the intervention was performed during the gestational period 12,15