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Most people relax comfortably during the session
Quantitative reverse transcription-polymerase chain reaction Gradient PCR was used to optimize the annealing temperature of gene-specific primers, identified from the literature or designed with primerBLAST ( Supplementary Table 1 )
Tested in WB, IHC, ICC/IF, Flow Cytometry applications
[DOI] [PMC free article] [PubMed] [Google Scholar] Wang Y., Zhang D., Zhang Y., Ni N., Tang Z., Bai Z., Shen B., Sun H., Gu P
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