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Glutathione reductase assay Following the Glutathione Reductase (GR) Assay Kit (ab83461, Abcam), twenty micrograms of cortical tissue from Wt and Tg animals was homogenized in 200 l of assay buffer on ice then sonicated twice in the span of five seconds and centrifuged at 10,000 g for 15 minutes (4 C)