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once those bonds are formed AP folds around them, stabilizing them against potential reductants during continued culture, cell harvesting, and extract preparation (Akiyama and Ito, 1993)
The cells were resuspended, and 10 L were loaded onto a cell microfluidic plate (Millipore-Sigma, CellASIC ONIX M04S-03) through the cell loading sequence for adherence overnight in 37 C, 5% CO2 incubator 42
