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250 L of 45 mM cystathionine (Sigma-Aldrich, Darmstadt, Germany) solution in 0.1 M phosphate buffer, pH 7.5 (2.5 mg of cystathionine per sample)

Values below the laboratory's lower limit generally indicate deficiency, while borderline results require clinical correlation

Thirdly, this reagent, as a powder is more stable, and can be stored at room temperature in brown glass bottle, while CMQT and CMLT require to be kept in darkness at 4 C

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Neuron-mediated generation of regulatory T cells from encephalitogenic T cells suppresses EAE