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The medication was designed for continued use, not discontinuation

Retatrutide is a triple-receptor agonist (GLP-1, GIP, and glucagon) that produced an average 24.2% body weight reduction over 48 weeks in Phase 2 clinical trials

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The mitochondrial suspension was then centrifuged at 8500 g for 20 min at 4 C and the remaining pellet was resuspended in TRIzol reagent (15596018, Invitrogen) for downstream extraction of mitochondrial RNA using Direct-zol RNA miniprep (R2055, Zymo) with DNase treatment