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doi: 10.1016/j.bbagen.2011.03.004

Peptides (100 M) corresponding to chymotryptic fragments from digested M pro (113:118, 127:134, 141:150, 155:159, 295: 305) were glutathionylated with 10 mM GSSG in 50 mM Tris-HCl buffer, 300 mM NaCl, and 2 mM EDTA pH 7.5 for 1 hour

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Tubes were vortexed for 510 s and incubated at room temperature for 5 min

and the hGH Biomarkers Test that is based on the measurement of IGF-1 and the N-terminal pro-peptide of type 3 collagen (P-III-P) as two markers shown to increase in a dose-dependent manner following GH administration [100]
