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4.1 In vitro experiments In a study conducted by An et al., using human umbilical vein endothelial cells, it was found that metformins antioxidant properties, at a dose of 2 mmol/L, were achieved through specific mechanisms

Such an increase in the mRNA levels of the catalytic subunit of -GCS was paralleled by a greater mucosal synthetic GSH capacity determined from either cysteine or -glutamylcysteine used as the sulfur aminoacid in the presence of glycine and glutamate and ATP as described (Fernndez-Checa and Kaplowitz, 1990), indicating an increase in the activity of -GCS following TNBS+ethanol administration (not shown)

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Since its discovery in 1973, in vivo and in vitro studies have shown that GHK-Cu possesses a wealth of health-positive actions including improving wound contraction and epithelization, and increasing the production of growth factors and activity of antioxidant enzymes

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