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67 l aliquot of the above solution was polarized in HyperSense polarizer (3.35T, 1.4 K, Oxford Instruments, UK) for ~1.5 hours and when maximal polarization was achieved, the sample was rapidly dissolved in 4 ml of isotonic buffer (40 mM Tris-HCl (pH = 8), 3 M ethylenediaminetetraacetic acid (EDTA), 22 M HCl) at physiological temperature