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Free Radic Biol Med 11:81128

Takeya R, Sumimoto H

Mild pain and swelling at the injection site can be eased by applying an ice pack for 1020 minutes as needed/when necessary

Finally, the protonated glutamate residue donates a hydrogen to the amine of the -linked peptide, releasing an amino acid with a free amino group (Figure 3) ( FIGURE 3 The ChaC-like family proteins, including CHAC1, preferentially degrade reduced glutathione but can also break down other -glutamyl amino acids ( Although CHAC2 enzymes in Leishmania major were shown to degrade only reduced and not oxidized glutathione, this specificity has yet to be confirmed for CHAC1 ( CHAC2 is both evolutionarily older and structurally distinct from CHAC1

While less specific to glutathione than Grx1-coupled sensors, rxYFP constructs have been used in compartment-specific redox measurements in preclinical models