glutathione synthetase deficiency prevalence Frontiers Early genetic diagnosis of glutathione
Description
4-HNE also regulates the Nrf2 pathway by binding to Keap1, its cytosolic inhibitor, promoting its release and allowing Nrf2 nuclear translocation
Matuszczak Y, Farid M, Jones J: Effect of n-acetylcysteine on glutathione oxidation and fatigue during handgrip exercise
An equal amount (1 g) of the total RNA for each sample was used as template to generate cDNA using iScript cDNA Synthesis Kit (Bio-Rad)
Mixtures were incubated with Glutathione Sepharose 4 Fast Flow resin (Cytiva) pre-equilibrated in Binding Buffer for 1 h at 4 C with overhead rotation in a 1 mL spin- column (MoBiTec)
Georg Thieme Verlag Stuttgart
