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Description
The reduced glutathione (GSH), as a key antioxidant, is synthesized exclusively in the cytosol through two ATP-dependent enzymes, namely -glutamyl-cysteine ligase (GCL), which is comprised of two independent subunits GCLC and GCLM

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The whole blood (30 mL) was layered on 20 mL Lymphoprep and centrifuged for 20 min at 400 g with the brake off, after which the upper plasma layer was removed and discarded
