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For histological examination, one side of the ovarian and uterine tissues was fixed in 4% paraformaldehyde, while the other side was quickly frozen in liquid nitrogen and stored at 80 C for later RNA extraction

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doi: 10.1016/j.nlm.2003.07.003
Finally, the tryptic peptides were desalted using SDB-RPS membrane (Sigma-Aldrich, 66886-U), vacuum-dried, and stored at 80 C before LC-MS/MS analysis
