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For transient transfection paradigms, 4 to 15 g of cDNA coding for N -terminally Flag-tagged WT human parkin or empty Flag control vector (pcDNA3.1) using a 1:1 ratio of cDNA:Lipofectamine 2000, was used for ectopic expression

Sustainable Dev

Glutathione also supports immune function by maintaining the balance of white blood cells and enhancing overall cellular defence mechanisms

cDNAs of tissues total RNA were synthesized using a PrimerScript TM RT reagent Kit with a gDNA Eraser (Perfect Real Time)(Takara, Japan) by S1000 TM Thermal Cycler (Bio-Rad, USA)

Protective packaging: Peptides are light- and temperature-sensitive