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The lysate was centrifuged at 10 000 g for 5 minutes at 4C and the supernatant was used for total glutathione estimation

We further evaluated the effect of GCLC succinylation on its enzymatic activity by measuring GSH synthesis rate in U2OS cells transfected with either Flag-tagged WT GCLC or its K-R and K-E mutants

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11): (i) overexpression of membrane transporter proteins, specifically ATP-binding cassette (ABC) proteins, which reduces drug influx and increases drug efflux in tumor cells

10.1016/S0021-9258(18)82124-X 55 JiA.-R.KuS.-Y.ChoM