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To simplify purification, affinity purification tags are often fused to a recombinant protein of interest.The most popular tag is the polyhistidine (6xHis) tag.Some proteins, like eukaryotic proteins may not exhibit proper protein activity or folding if expressed in E.coli

Shake the bottle well for 15-30 seconds until the powder is completely dissolved in the water

Here, the authors discover in lung cancer patients through redox proteomics that there is a higher oxidation of the cysteines of glycolytic and methylglyoxal metabolism enzymes.

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