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Secondary antibodies were diluted at 1/1000 in the saturation solution and incubated for 30 min at RT
Approximately 10 mL of phosphate-buffered isotonic solution (PBS, pH 7.4) was placed in the receiver phase, and 2 mL each of Lid and FD (Lid/AG = 1/1), dissolved in distilled water, was applied to the donor phase
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