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Colonies were selected and grown overnight in LB to extract plasmid DNA using a MiniPrep kit (NEB, Massachusetts, USA) using manufacturers protocol

doi: 10.1016/j.msec.2021.112233 73 LaxtonA

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During neuroinflammation, activated microglia can release stored iron, potentially contributing to oxidative stress in the surrounding tissue [12,14]

Pilot analysis of the plasma metabolite profiles associated with emphysematous Chronic Obstructive Pulmonary Disease phenotype
