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Lapenna D, de Gioia S, Ciofani G, et al
The mixture was then incubated for 15 min at 37 C, and after that, 5% TCA was added to halt the reaction
The embryos were then resuspended in blocking solution (0.5% BSA in PBS pH7.4) for 30 min at RT, followed by overnight incubation in primary antibodies at 4 C, and then a 2 h incubation in secondary antibodies (Invitrogen) at room temperature
2025;388(Pt 2):114351
This study is part of Chia-Yu Lais (first author) doctoral research project
