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For in vivo studies, a minimum of five mice per group was selected, which has been shown to be sufficient to detect biologically and statistically significant differences in similar experiments

Tao Y, Fu S, Lu J, Fu B, Liu S, Li L

Testosterone replacement therapy in chronic kidney disease patients

EJ Antibiot (Tokyo),1981 16

Whether the skin microbiome exerts a functional role in linking these two biological processes is still unclear
