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Livers were excised quickly, trimmed, rinsed in ice-cooled 0.9% (w/v) saline to remove blood, blotted dry and weighed at temperature of 0 to 4C

Always remember to remove air bubbles from the pin by flicking it gently, pin side up, and pushing bubbles out

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These adjustments allow marine mammals to optimize the use of their body oxygen stores while diving but can result in selectively reduced perfusion to peripheral tissues

The non-coenzyme action involves participation of thiamine and/or derivatives, including thiamine triphosphate, in the regulation of ACh synaptic function, consistent with the early data on thiamine as a co-mediator of ACh in neuromuscular synapses, and in allosteric action on metabolic enzymes