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We prepare the blood by placing it inside a centrifuge and separating the three constituent parts of blood, plasma, red blood cells and platelet rich plasma

Thus, many experts believe it to be a complex, multifactorial (including immune-mediated) disorder and perhaps one that encompasses several different hepatobiliary diseases

Sci Transl Med 2013;5:209ra151.ArticlePubMed 52

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Dermal fibroblast isolation All animals were anaesthetized with ketamine (75 mgkg 1 ) and xylazine (10 mgkg 1 ), and their dorsal hair was carefully removed