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these molecules harbour significant promise as therapeutics, or as early biomarkers during prodromal disease stages, or both

12-hour light-dark cycle) for at least one week prior to the experiment

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At 0, 3, 7, and 10 days, the tissues were fixed, and cryosections were used for in situ apoptotic staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), according to the manufacturers instructions (Servicebio, Beijing, China)