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Glutathione is a tripeptide synthesized at cytosolic level, that exists in cells in a reduced form (thiol-reduced-GSH-) and in an oxidized form (disulfide-oxidized)
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By quantifying how much of your protein was in your elution, and analyzing how much, if any, of your protein was in the flow-through, you can figure out if you used an appropriate amount of glutathione resin
