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According to the definitions used, cases had to have reduced their SPPB in at least 1 point, which is clinically relevant

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70 mL of 10 % HCl was added to the resulting concentrated solution which was then heated and stirred at a temperature of 80 to 90 0 C for 2 hours, concentrated by distillation under reduced pressure, and recrystallized from small amounts of anhydrous ethanol and acetone to afford L-carnitine hydrochloride