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10.1016/j.jphotobiol.2022.112508 92

The cryosections were washed with PBS and treated with methanol for 10 min, and then four times with 0.2% PBT (0.2% Triton X100 in PBS) for 10 min each time, and finally with Image-iT FX Signal Enhancer (Thermo Fisher Scientific) for 30 min

Gone, or at the very least, dramatically minimized

Water and black coffee are fine

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