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[DOI] [PMC free article] [PubMed] [Google Scholar] Warner LA, Sewell RL, Ma NS
The expression of UCP1, a crucial gene involved in thermogenesis, is also enhanced by ectopic expression of PGC-1 , which is critical for BAT thermogenesis [49, 51]
The gene for this has not been found in rabbits, nor any receptor or precursor peptide
For binding assays, 100 g of the purified GST-GOLPH3 fusion protein was first immobilized on 50 l of packed glutathione-agarose beads at room temperature for 1 h in TBST
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