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Ethical principles of the study complied with the European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13), the Animal Protection Act (Official Gazette 135/06), the ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), Federation of European Laboratory Animal Science Associations (FELASA) recommendations, and the recommendations of the Ethics Committee of the School of Medicine, University of Zagreb

1 Introduction Chronic kidney disease (CKD), characterized by progressive loss of renal function, has become a global public health problem that affects approximately 10% of the worlds population (Miguel et al., 2025

Some individuals may experience mild discomfort at the injection site or temporary redness

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