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The cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (12 10 5 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene

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This is likely because estrogen moderates the release of norepinephrine, reducing activation of the HPA axis during a stressful event

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