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Cells were washed in PBS and fixed in solution using 4% paraformaldehyde and 0.0075% glutaraldehyde in PBS for 25 minutes

Compound 482 was an interesting example of the latter

Over time, this chronic state of defensiveness may harden cell membranes, stop cells from communicating with each other, and cause cells to release ATP as a continued signal of cellular distress 2

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Initiation of pentostatin therapy in such patients can result in severe myelosuppression
