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High levels of stress were also reported to be connected with disruptions in serotonergic-related pathways, the volume of some brain areas such as the hippocampus and prefrontal cortex, and epigenetic changes in genes such as BDNF [49,50,51]

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The 2 CT method was used to determine the relative expression level of each gene [72]

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Micro-CT analysis revealed that DMM surgery induced severe osteophyte formation compared to sham controls, while intra-articular (IA) injection of Se/LDH@PEG-M decreased the osteophyte formation (Fig