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Dysregulation of the glutaredoxin/S- glutathionylation redox axis in lung diseases

The constant levels of marker proteins such as GAPDH, combined with observations indicating that knockdown of the UPR element, BiP/Grp78 , revealed no detectable effects on Nrf2, GCLC, or glutathione, further support the conclusion that NFT-mediated stabilization of Nrf2 is a major contributor to its accumulation and the subsequent stimulation of GCL and glutathione synthesis

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It can also be impaired by MTHFR genetic variations

The pyocyaninDNA binding mechanism was further confirmed using a Chirascan circular dichroism (CD) spectrophotometer (Applied Photophysics, UK)