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Nrf2 is the primary transcription factor that regulates antioxidant responses and inhibits ferroptosis in various cell types by protecting cells from lethal ROS stress (Gorrini et al., 2013

2.4 Sample preparation For LCQTOF-MS/MS analysis, 1 mg of powder or homogenized tablet was dissolved or extracted using 5 mL of a 1:1:1 (v/v/v) mixture of water/methanol/acetonitrile, assisted by ultrasonication for 10 min, and afterwards filtered by a Whatman 0.2 m pore size polytetrafluoroethylene (PTFE) filter medium (GE Healthcare, Chicago, IL, United States)

Deficiency can lead to macrocytic anaemia, neurological symptoms including peripheral neuropathy, cognitive changes, and fatigue

Its absence leads to reduced expression of glucose transporters GLUT1 and GLUT4 in the brain, and GLUT3 and GLUT4 in peripheral tissues