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Transfection of overexpressing or siRNA plasmids were carried out in 6070% confluent cells by using Lipofectamine 3000 reagent according to the manufacturers protocol

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However, at this time point, glutathione was elevated in both slim1-cr and 35S::SLIM1 plants ( Supplementary Figure 4B ), so it is unlikely to be a major contributor to the early bolting phenotype observed in the 35S::SLIM1 lines ( Figure 3A ) or directly related to the consistently greater size of 35S::SLIM1 plants across our 4-week time course ( Figure 1B )

Highcertainty evidence from up to 14 studies in around 3700 participants consistently shows a small clinical benefit for memantine versus placebo: clinical global rating (CGR): 0 Aarsland D, Ballard C, Walker Z, Bostrom F, Alves G, Kossakowski K, et al

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