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Finally, to confirm that the M105I mutation induces modifications in the -SNAP protein that alters its lipid binding affinity, and to rule out the possibility that the reduction in PM binding is due to differential interactions with membrane-associated proteins, we performed a liposome flotation assay
J Biol Chem 275:1553515540 Suzuki H, Kumagai H, Tochikura T (1986) Gamma-glutamyltranspeptidase from Escherichia coli K-12: formation and localization
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[DOI] [PubMed] [Google Scholar] 95.Kay C.D., Pereira-Caro G., Ludwig I.A., Clifford M.N., Crozier A
