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These receptors include the N-methyl-D-aspartate (NMDA) receptors and gamma-aminobutyric acid (GABA) receptors

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Protein extracts were separated on 8% SDS-polyacrylamide or 412% bistris protein gel (Invitrogen), transferred on a nitrocellulose membrane, and stained with antibodies against ZO-1 (N1N2, GeneTex, dilution 1:3000)

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