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Elongation factor 1 alpha (EF1) and -actin were used as the reference genes 61

Spermatogenesis assessment To evaluate spermatogenesis, at least 100 seminiferous tubules were examined in each animal and the quality of spermatogenesis in each tubule was scored according to the maturity of germ cells in the seminiferous tubules by Modified Johnsens score system

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Typical pooled effects over weeks to months are small - in the range of one to two kilograms - with stronger signals in people with overweight or obesity