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The specific target was the C32E8.9.1 transcript, with the goal of introducing a frameshift and a premature stop codon to disrupt its function

At day 3 of adulthood worms were picked, washed with PBS and stained with 5 M of dihydroethdium (DHE, Invitrogen) in PBS for 45 min at 20 C with agitation

[DOI] [PMC free article] [PubMed] [Google Scholar] 82.Pergola PE, Raskin P, Toto RD, Meyer CJ, Huff JW, Grossman EB, Krauth M, Ruiz S, Audhya P, Christ-Schmidt H, Wittes J, Warnock DG and Investigators BS

Higher crucifer intake correlates with reduced risk of colorectal, lung, and prostate cancer, although effect sizes vary by population and genetic background

Although GSH is rapidly oxidized to GSSG, the activity of e-GST remained unchanged, indicating a strong resistance to oxidants (Figure 3)