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Freeze or store on ice until ready to run SDS-PAGE

Effect of high dietary manganese intake of neonatal rats on tissue mineral accumulation, striatal dopamine levels, and neurodevelopmental status

To account for this potential confounder, we applied a linear mixed-effects model that included age, cohort, visit, and the interaction between cohort and visit, while also adjusting for principal components (PC1 and PC2) and patient-specific random effects

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