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Blood collection via caudal venipuncture was performed using heparinized syringes, with plasma isolated through centrifugation (4C, 3000 g, 15 min)

[DOI] [PMC free article] [PubMed] [Google Scholar] 16.Iwasa T., Matsuzaki T., Yano K., Mayila Y., Yanagihara R., Yamamoto Y., Kuwahara A., Irahara M

The reactions were performed in HPLC vials after adding the solutions as specified below

Biochem J 174(3):819825 Parsanathan R, Jain SK (2019) Glutathione deficiency induces epigenetic alterations of vitamin D metabolism genes in the livers of high-fat diet-fed obese mice

However, many women eventually stop having periods altogether, especially when using continuous combined HRT