ellman 1959 reduced glutathione Assay (Colorimetric) Ellman's Reagent - an overview

Cuprizone is a copper chelator widely used to cause specific oligodendrocyte cell death and demyelination, although its mechanism of action is incompletely understood 39,40

The C-terminal 6xHis-tagged LbcA protein was purified with HiTrap-Ni in 10 mM potassium phosphate, pH 8.0, 0.25 M NaCl, and a 20-CV 10300 mM imidazole gradient, followed by HiTrap-Q in 10 mM Tris, pH 8.0, and a 20-CV 50500 mM NaCl gradient

Potential Side Effects & Safety Considerations You've probably wondered if the serum's tiny drops could ever cause more trouble than they solve

This leads to the aggregation of copper-bound lipoylated mitochondrial proteins, which can disrupt the TCA cycle and, therefore, interfere with cellular energy production

doi: 10.1038/nrclinonc.2016.217