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The experiment was performed from 4:00 PM to 6:00 PM Oxidative stress assays Glutathione assay In order to measure the content of glutathione (GSH), the midbrain was removed and 10% tissue homogenates were mixed with an equal amount of 10% trichloroacetic acid

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Officials estimated that the sewer extensions alone would generate four thousand dollars per year in increased fees starting in 193 5.[253] Before workers completed the expansion, an awareness arose that the project would fail to achieve the citys revenue goals

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