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Rabbit sera were diluted in TBS/2% skimmed milk/20% sheep serum

PCA and Statistical Analysis Principal component analysis (PCA) was performed on mean centered data and statistical analyses were performed in R ( Projections were calculated by matrix multiplication of the centered data to be projected using the rotation matrix determined from the PCA of the original source data

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Streptavidin donor beads (40 g/mL) were further added for 1 h protected from light

However, after administration of necroptosis and ferroptosis inhibitors on top of the CDE diet, it was found that ferroptosis inhibitors almost completely reversed hepatocyte death, whereas necroptosis inhibitors failed to block the onset of cell death, suggesting that hepatocyte ferroptosis may be one of the initial modes of cell death at the onset of steatohepatitis and may be a trigger for the initial inflammation of steatohepatitis [135]