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Pharmalyte with an isoelectric point of 9.2 formed a buffering layer around MTW-immobilized enzyme, thus protecting it from surrounding bulk liquid with significant improvement in pH stability of the immobilized enzyme under alkaline conditions

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2b and Introduction) Power supply for electrophoresis and transfer device (e.g., PowerPac basic power supply, BioRad) Sonicator (e.g., Transonic T420 bath, CamLab) Temperature-controlled cabinet (e.g., Liebherr Profi line, Jencons) Temperature-controlled orbital shaker (e.g., Innova 4230 refrigerated benchtop incubator shaker, New Brunswick Scientific) Thermal cycler (e.g., PTC-150 Minicycler, MJ Research, GRI) Ultracentrifuge and rotor type 50.2 Ti (e.g., Optima L-100K, Beckman Coulter) UV transilluminator (e.g., UV transilluminator 2000, BioRad) XCell SureLock mini-cell (Cat