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Waiting 20 to 30 minutes before your morning coffee is a good rule of thumb.

( C ) ESR spectra of DEPMPO-trapped radicals, recorded from HeLa cells expressing APEX2 in either the cytosol (upper left trace) or the mitochondrial matrix (upper right trace), after exposure to HPI (1 mM), H 2 O 2 (1 mM) and DEPMPO (50 mM)

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doi:10.1016/j.molcel.2014.08.018 Zhao S, Lin Y, Xu W, Jiang W, Zha Z, Wang P, Yu W, Li Z, Gong L, Peng Y, Ding J, Lei Q, Guan KL, Xiong Y (2009) Glioma-derived mutations in IDH1 dominantly inhibit IDH1 catalytic activity and induce HIF-1alpha
