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[DOI] [PMC free article] [PubMed] [Google Scholar] 237.Yamamoto H, Schoonjans K, Auwerx J

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Lyophilized Storage Room temperature short-term, or 28C for long-term storage

In hepatocytes from rats fed diets with excess SAA, both overall catabolic flux and the proportion of Cys catabolism via cysteinesulfinate-dependent pathways increased markedly

Cycling was performed using an Eppendorf Mastercycler (Hamburg, Germany), as follows: a pre-PCR step of a 5-min denaturation at 94C, followed by 30 cycles of denaturation at 94C for 30 sec, annealing for 30 sec and extension at 72C for 30 sec and a final 5-min incubation at 72C