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Copper-catalyzed click-chemistry is performed under mild reaction conditions with stoichiometric amounts of each component (azide and alkyne building block) and with remarkable functional group compatibility 168

Mass Spectrometry (MS) confirms that the exact sequence of amino acids is present and correctly structured

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To do so, we measured intracellular levels of ROS in EJ cells with tetregulatable p21 expression (EJp21) by staining the cells with 2,7dichlorofluorescin diacetate (DCF), a fluorescent marker of cellular oxidant production (Royall and Ischiropoulos, 1993)