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Whole tick samples were homogenized using an automill (Tokken, Chiba, Japan) and were added to TRI Reagent (Sigma-Aldrich)

From a mechanistic perspective, primary protein carbonyls (i.e

In yeast, Hfd1 is required to oxidize 4-Hbz to 4-HB, and the human aldehyde dehydrogenase 3A1 (ALDH3A1) has also been shown to perform this step [37]

In order to test single cell line feasibility, each transfected cell line was first exposed to postulated Nrf2 inducers
