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The isopeptide bond in Spy0128-N is formed between Lys-36 on its first -strand (A) and Asn-168 on its last -strand (G), with the spontaneous intramolecular reaction being catalyzed by Glu-117 on the fifth -strand (E) 1

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References McEwen BS, et al

[DOI] [PMC free article] [PubMed] [Google Scholar] 25.Cheng N, He R, Tian J, Dinauer MC, and Ye RD 2007

The finding that a GHRH analogue and a growth-hormone-releasing peptide combine to produce a supra-additive GH pulse goes back to work from around 1990 onward, and it is robust 4 but it is a finding about the two classes , demonstrated with various specific peptides, not a study of this exact pair